The effect of climate index on growth values from birth to breeding in Akkaraman sheep.

Under specific agroclimatic conditions, growth is an adaptability parameter resulting in reduced performance with decreasing animal comfort of a sheep breed. In this study, a total of 1011 lambs' live weights (LW) and average daily weight gains (ADG) in 16 different farms between 2018 and 2020 (birth to 18 months) were determined in Akkaraman herds raised in Çankiri Province of Turkey. Comprehensive Climate Index (CCI) was examined to determine the effect of climate. Thermal comfort limits in CCI values were classified as CCI-1.cold (≤ 5 ℃), CCI-2.comfort (5-25 ℃), and CCI-3.warm (≥ 25 ℃). The climatic values that all lambs examined were exposed to according to index value groups in periods from birth to 18 months of age were prepared daily. The lambs were exposed to comfort environment in the majority of the days in the MP0-3 (77.7%) and MP12-18 (50.1%) periods. The lambs mostly in the MP3-6 period (72.2%) were above the warm stress threshold, but in the MP6-12 period, 54.7% were above the cold stress threshold. Overall mean LW of lambs at birth, at 3, 6, 12, and 18 months were 4.29 kg, 35.1 kg, 39.1 kg, 45.4 kg, and 52.6 kg, while the ADG values between birth-3 months, 3-6 months, 6-12 months, and 12-18 months were 341.8 g, 54.5 g, 42.9 g, and 56.8 g respectively. Periodically, CCI values were realized as 10-15, 25-30, and 18-22 in the growth periods of 0-3, 3-6, and 12-18 months, respectively, and ADG values of the lambs were found to be similar within their own periods. However, when the CCI values of the lambs increase to 5 and above in the 06-12-month period, there is a decrease in their live weight. As a result, climatic index values did not have a negative effect on the growth and development values of lambs in cold conditions in general, but warm conditions affected the animals negatively.

Using ANI 35/L approach to evaluate the welfare status of locally adapted Anatolian Black cattle.

In this study, it was aimed to determine of the shelter conditions and welfare levels of Anatolian Black cattle grown in two different regions of Turkey. Accordingly, welfare assessment was made at the farm level according to the Animal Needs Index (ANI 35L/2000) method, a total of 76 in Ankara and Antalya provinces. The material of the research was composed of Anatolian Black cattle farms within the scope of the Project on Conservation of Domestic Genetic Resources. According to the ANI welfare assessment method, scoring was composed of a total of 30 criteria in 5 different categories, namely, locomotion, social interaction, flooring, light air and stockmanship. In the study, the average of all farms was determined as 22.73 points in winter and 41.36 points in summer. These values were found to be 22.00 and 23.98 in winter and 40.87 and 42.20 in summer, respectively, in Ankara and Antalya provinces. As a result of the study, it was found that the opportunity for animals to go to the courtyard and pasture has positive contributions to animal welfare. Moreover, it determined that factors such as stall size, available floor area, flooring and lighting adversely affected animal welfare in winter. The ANI 35L method can be recommended to determine the welfare levels of farms that have production by traditional methods and not possible to examine many animal-based parameters.

Determination of the relationship between Anatolian black cattle growth properties and myostatin, GHR and Pit-1 gene.

The aim of this study is to determine the relationship between some growth and development characteristics in Anatolian Black cattle from birth to twelve months of age with the Pit-1, GHR and Myostatin genes. PCR-RFLP method was used to detect the polymorphism. Genotype and allele frequencies were found to be AA/AB/BB: 0.096/0.519/0.385 and A/B: 0.356/0.644; AA/AG/GG: 0.346/0.385/0.269 and A/G: 0.538/0.462 in the Pit-1 and GHR genes respectively. Myostatin gene was found to be also monomorphic in all genotypes. Although the chi-square χ2 test in the Pit-1 gene showed an agreement to Hardy-Weinberg equilibrium (p > 0.05), in the GHR gene did not showed an agreement (p < 0.05). The results of the statistical analysis indicated an association between Pit-1 and GHR genes polymorphism and growth traits at different stage ages in Anatolian Black cattle. But Pit-1/HinfI gene and GHR/Alul polymorphisms were not found statistically significant in the specified periods, at all characters. On the other hand, since the MSTN/BstF5I gene was found to be monomorphic, no association analysis was performed between the measured values and this gene. In conclusion, mutation of these genes is difficult to suggest as a potential marker in a herd selection regarding the growth and development characteristics.

Association between MYLK4 gene polymorphism and growth traits at different age stages in Anatolian black cattle.

This study aims to find the association between MYLK4 gene polymorphism and growth traits at different ages, such as birth, 3rd-month, 6th-month and 12th-month ages in Anatolian black cattle. PCR-RFLP method was used to detect the polymorphism for MYLK4 gene in at G61595A locus. Allele and genotype frequencies were A: 0.34, G: 0.66 and AA: 0.17, AG: 0.34 and GG: 0.49. The chi-square χ2 test showed an agreement to Hardy-Weinberg equilibrium (p > .01). GLM and RRM models were used to estimate the association between MYLK4 gene polymorphism and growth traits. The results of the statistical analysis indicated an association between MYLK4 gene polymorphism and growth traits at different stage ages in Anatolian black cattle. Therefore, the G61595A mutation of MYLK4 gene can be used as a genetic marker for the selection of growth and development traits in the cattle breeding programs.